TMRM-Perchlorate-solution-生命科學(xué)試劑-MCE

Hotline:400-820-3792Inhibitors?ScreeningLibraries?Proteinswww.MedChemETMRMPerchlorate(solution)Cat.No.:HY-DY1042CASNo.:115532-50-8分?式:C??H??ClN?O?分?量:500.93作?靶點:FluorescentDye作?通路:Others儲存?式:-20°C,protectfromlight.Avoidrepeatedfreezingandthawing*Insolvent:-80°C,6months;-20°C,1month(protectfrom

light.Avoidrepeatedfreezingandthawing)BIOLOGICALACTIVITY?物活性羅丹明類染料?種膜滲透性的陽離?熒光探針，可對線粒體膜電位進?特異性識別，從?附在線粒體中，并產(chǎn)?明亮熒光，在?定濃度下，羅丹明類染料對細胞具有低毒性，因此遍?于檢測動物細胞、植物細胞以及微?物中的線粒體。溶劑及濃度：DMSO：10mM體外研究1.PreparationofTMRMworkingsolutionDilutethestocksolutioninserum-freecellculturemediumorPBS.Thecorrespondingstocksolutioncanbedilutedaccordingtotheactualsituation.NotethatifthesolventisDMSO,thecytotoxicityofDMSOmustbeconsidered,andasolventcontrolshouldbeprepared;ifthesolventispurewater,theworkingsolutionneedstobefilteredandsterilizedbeforeaddingcells.Note:PleaseadjusttheconcentrationofTMRMworkingsolutionaccordingtotheactualsituation.2.Cellstaining2.1Suspensioncells(6-wellplate)a.Centrifugeat1000gat4°Cfor3-5minutesandthendiscardthesupernatant.WashtwicewithPBS,5minuteseachtime.Thecelldensityis1×106/mL.b.Add1mLofworkingsolution,andthenincubateatroomtemperaturefor5-30minutes.c.Centrifugeat400gat4°Cfor3-4minutesandthendiscardthesupernatant.d.WashtwicewithPBS,5minuteseachtime.e.Resuspendcellswithserum-freecellculturemediumorPBS.Observationbyfluorescencemicroscopyorflowcytometry.2.2Adherentcellsa.Cultureadherentcellsonsterilecoverslips.1/2MasterofBioactiveMolecules—您?邊的抑制劑?師www.MedChemEb.Removethecoverslipfromthemediumandaspirateexcessmedium.c.Add100μLofworkingsolution,gentlyshakeittocompletelycoverthecells,andthenincubateatroomtemperaturefor30-60minutes.d.Washtwicewithmedium,5minuteseachtime.Observationbyfluorescencemicroscopyorflowcytometry.Note:Ifdetectionbyflowcytometry,cellsneedtoberesuspendedbeforestaining.REFERENCES[1].CrowleyLC,etal.MeasuringMitochondrialTransmembranePotentialbyTMREStaining.ColdSpringHarbProtoc.2016Dec1;2016(12):t087361.[2].ChowdhurySR,etal.Simultaneousevaluationofsubstrate-dependentoxygenconsumptionratesandmitochondrialmembranepotentialbyTMRMandsafraninincorticalmitochondria.BiosciRep.2015Dec8;36(1):e00286.[3].MonteithA,etal.Imagingofmitochondrialandnon-mitochondrialresponsesinculturedrathippocampalneuronsexposedtomicromolarconcentrationsofTMRM.PLoSOne.2013;8(3):e58059.McePdfHeightCaution:Producthasnotbeenfullyvalidatedformedicalapplicati